Composite

Part:BBa_K5416071:Design

Designed by: TIANLANG CHEN   Group: iGEM24_Imperial-College   (2024-09-30)


IC240401_pQE60-DXS-IspH


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 38
    Illegal EcoRI site found at 1127
    Illegal XbaI site found at 3011
    Illegal PstI site found at 848
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 38
    Illegal EcoRI site found at 1127
    Illegal PstI site found at 848
    Illegal NotI site found at 440
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 38
    Illegal EcoRI site found at 1127
    Illegal BamHI site found at 6529
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 38
    Illegal EcoRI site found at 1127
    Illegal XbaI site found at 3011
    Illegal PstI site found at 848
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 38
    Illegal EcoRI site found at 1127
    Illegal XbaI site found at 3011
    Illegal PstI site found at 848
    Illegal NgoMIV site found at 919
    Illegal AgeI site found at 398
    Illegal AgeI site found at 977
    Illegal AgeI site found at 6364
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1889
    Illegal BsaI.rc site found at 4384
    Illegal SapI site found at 3301
    Illegal SapI site found at 6346
    Illegal SapI.rc site found at 782
    Illegal SapI.rc site found at 1625


Design Notes

This part is integrated with DXS(BBa_K118000) for achieving IPP overexpression under arabinose induction.


Source

E.coli

References