Composite
Part:BBa_K5416071:Design
Designed by: TIANLANG CHEN Group: iGEM24_Imperial-College (2024-09-30)
IC240401_pQE60-DXS-IspH
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 38
Illegal EcoRI site found at 1127
Illegal XbaI site found at 3011
Illegal PstI site found at 848 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 38
Illegal EcoRI site found at 1127
Illegal PstI site found at 848
Illegal NotI site found at 440 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 38
Illegal EcoRI site found at 1127
Illegal BamHI site found at 6529 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 38
Illegal EcoRI site found at 1127
Illegal XbaI site found at 3011
Illegal PstI site found at 848 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 38
Illegal EcoRI site found at 1127
Illegal XbaI site found at 3011
Illegal PstI site found at 848
Illegal NgoMIV site found at 919
Illegal AgeI site found at 398
Illegal AgeI site found at 977
Illegal AgeI site found at 6364 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1889
Illegal BsaI.rc site found at 4384
Illegal SapI site found at 3301
Illegal SapI site found at 6346
Illegal SapI.rc site found at 782
Illegal SapI.rc site found at 1625
Design Notes
This part is integrated with DXS(BBa_K118000) for achieving IPP overexpression under arabinose induction.
Source
E.coli